Table 1.
Efficiency of germ-line transmission and transgenic chick production for G418-selected donor PGCs
| ID no. | Period of G418-selection (passage) | No. of hatched chicks | No. of endogenous germ-cell–derived chicks* (%) | No. of donor germ-cell–derived chicks† (%) | No. of transgenic chicks (%)‡ |
| 9154B | 45 d (p46) | 63 | 4 (6.3) | 59 (93.7) | 32 (54.2) |
| 9155B | 45 d (p46) | 76 | 2 (2.6) | 74 (97.4) | 36 (48.6) |
| 9173B | 54 d (p47) | 102 | 5 (4.9) | 97 (95.1) | 51 (52.6) |
| 9176B | 54 d (p47) | 52 | 5 (9.6) | 47 (90.4) | 23 (48.9) |
| 9186B | 67 d (p49) | 89 | 1 (1.1) | 88 (98.9) | 45 (51.1) |
| 9187B | 67 d (p49) | 77 | 5 (6.5) | 72 (93.5) | 41 (56.9) |
| Total | 459 | 22 (4.8) | 437 (95.2) | 228 (52.2) |
*Test-cross analysis was conducted by mating between KO (i/i) and germ-line chimeric KO (i/i); that is, transplanted GFP-expressing donor PGCs of WL (I/I). The phenotype of endogenous recipient germ-cell–derived chick is black KO (i/i).
†The phenotype of offspring derived from donor GFP+ PGCs of WL (I/I) is white hybrid (I/i) between KO (i/i) and WL (I/I).
‡The percentage of GFP-expressing transgenic chicks in donor germ-cell–derived hybrid chicks.