Nanos and Bam protein expression in ovaries, testis, and snf148 ovarian tumors. (A–C) Confocal images of gonads from WT animals and tumorous ovaries from snf148/snf148 females carrying fully functional copies of a Bam-GFP fusion protein and a Nanos-Myc fusion protein costained for GFP and MYC. (A) In the ovary, Bam and Nanos protein are expressed in nonoverlapping domains in early germ cells. The GSCs are located adjacent to the somatic cap cells, marked with an asterisk. Domains are numbered as in D. (B) In the testis, Nanos and Bam are coexpressed in early germ cells, except for the presumptive GSCs located adjacent to the hub, marked with an asterisk. (C) In the tip of the snf148/snf148 ovarian tumor, Nanos and Bam are coexpressed, except for the presumptive GSCs located adjacent to the somatic cap cells marked with an asterisk. Images in A–C are the same magnification.