Fig. 3.

EPO induces Egr2 mRNA in rat B104-EPOR neuronal cells in vitro. Cells were plated in 24-well plates at 120,000 cells/mL in complete medium. After overnight incubation, the cells were deprived of serum for 4 h and then were stimulated with EPO (80 ng/mL) for the indicated time. Egr2 mRNA was measured by qPCR, using GAPDH as a housekeeping gene. Results represent the change in expression level vs. one of the control (serum) samples, expressed as log base 2 ratio, and are the mean ± SD of triplicate samples assayed in duplicate. ***P < 0.001; *P < 0.05 vs. no serum by Student’s t test.