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Mutagenesis analysis of nsp14 ExoN activity. Residues from the nsp14 ExoN catalytic site and from the SAM-binding site of the nsp14 MTase domain were mutated into alanine. Equal amounts of each nsp14 mutant were incubated with nsp10 and *p-H4 RNA for 0, 2, and 30 min. In lane 5XD331A, the concentration of the mutant was fivefold higher. The panel shows the time-dependent hydrolysis of *p-H4 RNA after Urea-PAGE separation and autoradiography.
