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The nsp10/nsp14 ExoN activity is a dsRNA-dependent exonuclease. Autoradiogram of an ExoN assay. HPLC-purified ssRNA (RNA11) was labeled at its 5′-end using PNK and [γ32P]ATP, and hybridized to its complementary strand modified at its 3′-end with biotin (RNA11revbiot). Single- or double-stranded labeled-RNAs were subjected to nsp10/nsp14 ExoN digestion for 0, 2, and 30 min. Digestion products were separated on Urea-PAGE and revealed by autoradiography.
