Skip to main content
. 2012 May 21;109(24):9426-9431. doi: 10.1073/pnas.1201781109

Fig. 2.

Fig. 2.

Dbp5 within the cell. (A) Fluorescence of C.tentans Dbp5-AlexaFluor647 after microinjection into the cytoplasm of salivary gland cells imaged after 20 min incubation in hemolymph. (B) Fluorescence of coinjected BSA-AlexaFluor546. BSA cannot pass the NE, thus marking the injection site and demonstrating NE integrity. (C) Distribution of Dbp5 coinjected with actinomycin D, (D) distribution of coinjected BSA. Images shown in (C) and (D) were taken after approximately 20 min incubation of the gland in hemolymph. (E) Distribution of the Dbp5(R252A) point mutant after cytoplasmic injection. The highly conserved arginine at position 252 of C.tentans Dbp5 (corresponding to R259 in the human Dbp5 ortholog DDX19) mediates a critical salt bridge to NUP214. After replacing the arginine by alanine, the Dbp5-NPC interaction is almost completely lost. This point mutant showed the same subcellular distribution as WT Dbp5 after transcription blocking.