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. 2012 May 29;109(24):9360–9365. doi: 10.1073/pnas.1121607109

Fig. 2.

Fig. 2.

Mapping the interactions between TisAFP6 and ice. (A) Locations of mutated T82, N178, and V221 in the center of the a–c faces, respectively. (B) Plot of TH vs. protein concentration for the three mutants and wild-type TisAFP6 tagged with GST. (C) FIPA pattern obtained with fluorescently tagged TisAFP6–V221C mutant (without GST) on a single ice-crystal hemisphere mounted with the c-axis along the ice finger. (D) Interpretive illustration of C, indicating the three a-axes relative to the six equivalent prism planes bound by TisAFP6. The basal plane is central underneath the hexagonal pit used to deduce the direction of the a-axes (E) FIPA pattern obtained with TisAFP6 from a different angle. (F) Interpretive illustration of E showing that the six equivalent bound prism planes are distinct from both the primary (1°) and secondary (2°) prism planes.