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. 2012 May 30;109(24):9593–9598. doi: 10.1073/pnas.1202910109

Fig. 2.

Fig. 2.

Inhibition of LMP1 through a DN or with shRNAs. (A) LMP1 expression in stable cell lines expressing a DN LMP1 (204-208AAAAA, Y384G), the pBABE vector control, two independent shRNAs targeted to LMP1, and a scrambled shRNA sequence used as a negative control. Shown is a long-exposure Western blot for LMP1, illustrating the large degree of overexpression of the DN construct over the endogenous LMP1 and knockdown by the shRNAs. GAPDH is used as a loading control. (B) Stable cell lines were grown in soft agar for 12 d. Arrows point to examples of the four classes of colonies scored below; single cell (1), aborted colony (2), small colony (3), and large colony (4). The pictures as currently shown are at 50× magnification. (C) Colonies in each soft agar assay were counted and classified based on the four categories shown above. At least 150 colonies for each genotype were counted.