FIG. 1. .

Stimulation of Fam110c expression by hCG in whole ovaries and granulosa cells in vivo. Real-time PCR analysis shows expression of Fam110c mRNA in periovulatory ovaries (A) and granulosa cells in vivo (B) after hCG administration. eCG-primed rats were injected with hCG, and ovaries or granulosa cells were collected at 0, 4, 8, 12, or 24 h after treatment. Relative levels of Fam110c mRNA were normalized to those of Rpl32 in each sample (mean ± SEM; n = 3 independent culture experiments). Western blot analysis shows FAM110C protein levels at different time points after hCG treatment in intact rat ovaries (C) and granulosa cells in vivo (D). Upper panel shows a representative Western blot; lower panel shows relative quantitative levels of FAM110C protein normalized to that of β-actin in each sample (mean ± SEM; n = 3 independent culture experiments). Bars with no common letters are significantly different (P < 0.05).