FIG. 4. .

Activity of Fam110c promoter reporter constructs in cultured granulosa cells. Granulosa cells isolated from preovulatory ovaries (48 h after eCG) were transiently transfected with empty luciferase reporter vector (LUC), bp fragments −874/+26, −516/+26bp, −138/+26, and −138/+26, Sp1 mutant, or bp fragment −49/+26 Fam110c promoter luciferase reporter constructs and treated with FSK (10 μM) plus PMA (20 nM) for 6 h. Firefly luciferase activity was normalized to Renilla luciferase activity. Each experiment was performed in triplicate, and the experiment was repeated at least three times. Bars with no common letters within a reporter construct are significantly different (P < 0.05).