FIG. 5. .

Analysis of granulosa cell cycle kinetics after overexpression of rat FAM110C. Granulosa cells from preovulatory ovaries were infected with either Ad-Fam110c or an Ad-GFP control adenovirus. A) Western blot analysis demonstrates expression of FAM110C and the endogenous control β-actin. Upper panel shows a representative Western blot; lower panel shows relative quantitative levels of FAM110C protein normalized to β-actin in each sample. B) Cell cycle kinetics using flow cytometry. Data demonstrate percentages of cells in different stages of cell cycles, G₁ (G₀–G₁), G₂, and S phases, in cells overexpressing FAM110C. Results represent means ± SEM for at least 3 individual experiments. Bars that do not share a letter or number designation are significantly different (P < 0.05).