FIG. 3. .

In situ hybridization analysis of Rhox8 expression in the adult epididymis. WT and Rhox5-null epididymal samples (n = 6) were prepared from littermates that were coprocessed and developed for the same lengths of time. Rhox8 mRNA was detected by antisense probe in the initial segment (IS), caput, corpus, and cauda (numbers correspond to previously characterized segments of the mouse epididymis [31]). No significant signal was observed with the sense probe in any region. Original magnification ×200.