Table 1. DNA methylation changes in shoot and root at seedling stage under non-stress and salinity stress conditions.
IR29 | Nipponbare | Pokkali | Geumgangbyeo | |||||||
MSAP band type | Control | Salinity | Control | Salinity | Control | Salinity | Control | Salinity | ||
A. Shoot | ||||||||||
I | 665 | 789 | 877 | 692 | 698 | 566 | 838 | 1067 | ||
II | 201 | 113 | 246 | 290 | 293 | 317 | 143 | 119 | ||
III | 322 | 425 | 205 | 235 | 171 | 158 | 160 | 93 | ||
IV | 446 | 308 | 306 | 418 | 471 | 593 | 489 | 350 | ||
Total amplified bands | 1634 | 1635 | 1634 | 1635 | 1633 | 1634 | 1630 | 1629 | ||
MSAP (%)a | 59.3 | 51.7 | 46.4 | 57.6 | 57.2 | 65.3 | 48.6 | 34.5 | ||
Fully methylated band (%)b | 47.0 | 44.8 | 31.3 | 39.9 | 39.3 | 45.9 | 39.8 | 27.2 | ||
Hemi-methylated band (%)c | 12.3 | 6.9 | 15.1 | 17.7 | 17.9 | 19.4 | 8.8 | 7.3 | ||
B. Root | ||||||||||
I | 1276 | 1314 | 1103 | 1310 | 1231 | 1232 | 1311 | 1319 | ||
II | 7 | 23 | 34 | 39 | 32 | 34 | 10 | 19 | ||
III | 98 | 84 | 239 | 83 | 91 | 114 | 95 | 97 | ||
IV | 254 | 211 | 259 | 203 | 232 | 202 | 218 | 197 | ||
Total amplified bands | 1635 | 1632 | 1635 | 1635 | 1586 | 1582 | 1634 | 1632 | ||
MSAP (%)a | 21.9 | 19.5 | 32.6 | 19.9 | 22.4 | 22.1 | 19.8 | 19.2 | ||
Fully methylated band (%)b | 21.5 | 18.1 | 30.5 | 17.5 | 20.4 | 19.9 | 19.2 | 18.0 | ||
Hemi-methylated band (%)c | 0.4 | 1.4 | 2.1 | 2.4 | 2.0 | 2.2 | 0.6 | 1.2 |
MSAP (%) = [(II+III+IV)/(I+II+III+IV)]×100;
Fully methylated bands (%) = [(III+IV)/(I+II+III+IV)]×100;
Hemimethylated bands (%) = [(II)/(I+II+III+IV)]×100.
Type I indicated absence of methylation due to the presence of bands in both EcoRI/HpaII and EcoRI/MspI digest; type II bands appeared only in EcoRI/HpaII digestion but not in the EcoRI/MspI digest; type III generated bands obtained in EcoRI/MspI digest but not in the EcoRI/HpaII digest; and type IV represents the absence of band in both enzyme combinations.