Table 1. DNA methylation changes in shoot and root at seedling stage under non-stress and salinity stress conditions.

	IR29		Nipponbare			Pokkali			Geumgangbyeo
MSAP band type	Control	Salinity	Control	Salinity		Control		Salinity	Control	Salinity
A. Shoot
I	665	789	877	692		698		566	838	1067
II	201	113	246	290		293		317	143	119
III	322	425	205	235		171		158	160	93
IV	446	308	306	418		471		593	489	350
Total amplified bands	1634	1635	1634	1635		1633		1634	1630	1629
MSAP (%)a	59.3	51.7	46.4	57.6		57.2		65.3	48.6	34.5
Fully methylated band (%)b	47.0	44.8	31.3	39.9		39.3		45.9	39.8	27.2
Hemi-methylated band (%)c	12.3	6.9	15.1	17.7		17.9		19.4	8.8	7.3
B. Root
I	1276	1314	1103		1310		1231	1232	1311	1319
II	7	23	34		39		32	34	10	19
III	98	84	239		83		91	114	95	97
IV	254	211	259		203		232	202	218	197
Total amplified bands	1635	1632	1635		1635		1586	1582	1634	1632
MSAP (%)a	21.9	19.5	32.6		19.9		22.4	22.1	19.8	19.2
Fully methylated band (%)b	21.5	18.1	30.5		17.5		20.4	19.9	19.2	18.0
Hemi-methylated band (%)c	0.4	1.4	2.1		2.4		2.0	2.2	0.6	1.2

^aMSAP (%)  =  [(II+III+IV)/(I+II+III+IV)]×100;

^bFully methylated bands (%) = [(III+IV)/(I+II+III+IV)]×100;

^cHemimethylated bands (%) = [(II)/(I+II+III+IV)]×100.

Type I indicated absence of methylation due to the presence of bands in both EcoRI/HpaII and EcoRI/MspI digest; type II bands appeared only in EcoRI/HpaII digestion but not in the EcoRI/MspI digest; type III generated bands obtained in EcoRI/MspI digest but not in the EcoRI/HpaII digest; and type IV represents the absence of band in both enzyme combinations.