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. 2012 Jun 28;7(6):e39761. doi: 10.1371/journal.pone.0039761

Figure 6. TGFβ signaling in primary mouse fibroblasts from different sites of lung.

Figure 6

A. Expression of phosphorylated and nonphosphorylated Smad2 protein, as a measure of TGFβ signaling, and Smad4 in primary fibroblasts. B. Densitometry for pSmad2/Smad2 and Smad4. Ratios of pSmad2/Smad2 are higher in PF compared to LAF and MAF and are increased by cigarette smoke as well as exogenous TGFβ. The findings suggest that PF have higher levels of intrinsic TGFβ signaling and that smoke leads to TGFβ release. C-Control - fibroblasts from animals exposed to air; S- Smoke - fibroblasts from animals exposed to cigarette smoke. Data are mean+/−SD.