TABLE 1:
Yeast strains used in this study. Continued
| Strain | Genotype | Source |
|---|---|---|
| J230‑2D | a dbf2‑1 dbf20∆::TRP1 ade1 leu2 trp1 ura3 | Toyn and Johnston (1994) |
| YEF473 | a/α his3/his3 leu2/leu2 lys2/lys2 trp1/trp1 ura3/ura3 | Bi and Pringle (1996) |
| YEF473A | a his3 leu2 lys2 trp1 ura3 | Bi and Pringle (1996) |
| YEF473B | α his3 leu2 lys2 trp1 ura3 | Bi and Pringle (1996) |
| YEF2845 | As YEF473 except chs2∆::His3MX6/CHS2 | This studya |
| YEF6653 | a CHS2-GFP::His3MX6 CDC3-mCherry::URA3 | This study |
| YO790 | a chs1∆::KanMX6 chs3∆::His3MX6 | This studya |
| YO1111 | a chs1∆::NatMX6 chs3∆::His3MX6 | This study |
| YO1172 | a/α chs2∆::His3MX6/CHS2 chs3∆::KanMX6/CHS3 [pEC2, 2 μ, CHS2, URA3] | This study |
| YO1182 | a CHS2-GFP::KanMX6 DBF2-mCherry::His3MX6 | This study |
| YO1194 | α chs2∆::His3MX6 [pEC2, 2 μ, CHS2, URA3] | This study |
| YO1209 | a chs2∆::His3MX6 cyk3∆::TRP1 leu2::LEU2‑pRS305 | This study |
| YO1210 | a chs2∆::His3MX6 cyk3∆::TRP1 CHS2-GFP::KanMX6::LEU2 | This study |
| YO1211 | a chs2∆::His3MX6 cyk3∆::TRP1 chs2-S217A S225A-GFP::KanMX6::LEU2 | This study |
| YO1212 | a chs2∆::His3MX6 cyk3∆::TRP1 chs2-S217D S225D-GFP::KanMX6::LEU2 | This study |
| YO1213 | a chs2∆::His3MX6 leu2::LEU2‑pRS305 [pBK42, 2 μ, CYK3, TRP1] | This study |
| YO1214 | a chs2∆::His3MX6 CHS2-GFP::KanMX6::LEU2 [pBK42, 2 μ, CYK3, TRP1] | This study |
| YO1215 | a chs2∆::His3MX6 chs2-S217A S225A-GFP::KanMX6::LEU2 [pBK42, 2 μ, CYK3, TRP1] | This study |
| YO1216 | a chs2∆::His3MX6 chs2-S217D S225D-GFP::KanMX6::LEU2 [pBK42, 2 μ, CYK3, TRP1] | This study |
| YO1129 | a chs1∆::KanMX6 chs3∆::His3MX6 [p414ADH, CEN, TRP1] | This study |
| YO1130 | a chs1∆::KanMX6 chs3∆::His3MX6 [pBK42, 2 μ, CYK3, TRP1] | This study |
| YO1244 | a chs1∆::TRP1 chs2∆::His3MX6 chs3∆::KanMX6 [pEC2, 2 μ, CHS2, URA3] | This study |
| YO1245 | a chs2∆::His3MX6 leu2::LEU2‑pRS305 [pRS314, CEN, TRP1] | This study |
| YO1246 | a chs2∆::His3MX6 CHS2-GFP::KanMX6::LEU2 [pRS314, CEN, TRP1] | This study |
| YO1247 | a chs2∆::His3MX6 chs2-S217A S225A-GFP::KanMX6::LEU2 [pRS314, CEN, TRP1] | This study |
| YO1248 | a chs2∆::His3MX6 chs2-S217D S225D-GFP::KanMX6::LEU2 [pRS314, CEN, TRP1] | This study |
| YO1320 | a chs1∆::TRP1 chs2∆::His3MX6 chs3∆::KanMX6 [YCp111-CHS2-GFP::KanMX6, CEN, LEU2] | This study |
| YO1324 | a chs1∆::TRP1 chs2∆::His3MX6 chs3∆::KanMX6 [YCp111-CHS2-S217A‑GFP::KanMX6, CEN, LEU2] | This study |
| YO1325 | a chs1∆::TRP1 chs2∆::His3MX6 chs3∆::KanMX6 [YCp111-CHS2-S217D-GFP::KanMX6, CEN, LEU2] | This study |
| YO1338 | α chs2∆::His3MX6 CDC3-mCherry::TRP1 leu2::LEU2‑pRS305 | This study |
| YO1339 | α chs2∆::His3MX6 CDC3-mCherry::TRP1 CHS2-GFP::KanMX6::LEU2 | This study |
| YO1359 | α chs2∆::His3MX6 CDC3-mCherry::TRP1 chs2‑S217A-GFP::KanMX6::LEU2 | This study |
| YO1360 | α chs2∆::His3MX6 CDC3-mCherry::TRP1 chs2‑S217D-GFP::KanMX6::LEU2 | This study |
| YO1398 | a dbf2‑1 dbf20∆::TRP1 CDC3‑mCherry::LEU2 | This studyb |
| YO1399 | a dbf2‑1 dbf20∆::TRP1 CDC3‑mCherry::LEU2 pGAL1‑SIC1s (T5V, T33V, S76A)‑HA::URA3 [YCp111‑CHS2‑GFP::KanMX6, CEN, LEU2] | This study |
| YO1400 | a dbf2‑1 dbf20∆::TRP1 CDC3‑mCherry::LEU2 pGAL1‑SIC1s (T5V, T33V, S76A)‑HA::URA3 | This study |
| YO1410 | a dbf2‑1 dbf20∆::TRP1 CDC3‑mCherry::LEU2 pGAL1‑SIC1s (T5V, T33V, S76A)‑HA::URA3 [YCp111‑CHS2‑S217A‑GFP::KanMX6, CEN, LEU2] | This study |
| YO1411 | a dbf2‑1 dbf20∆::TRP1 CDC3‑mCherry::LEU2 pGAL1‑SIC1s (T5V, T33V, S76A)‑HA::URA3 [YCp111‑CHS2‑S217D‑GFP::KanMX6, CEN, LEU2] | This study |
| YO1450 | α chs2∆::HIS3 CDC3-mCherry::TRP1 chs2-S217A S225A-GFP::KanMX6::LEU2 | This study |
| YO1451 | α chs2∆::HIS3 CDC3-mCherry::TRP1 chs2-S217D S225D-GFP::KanMX6::LEU2 | This study |
| YO1454 | α chs2∆::His3MX6 CHS2-GFP::KanMX6::LEU2 [pRS314-MYO1-C-mCherry, CEN, TRP1] | This study |
| YO1453 | α chs2∆::His3MX6 chs2-S217A S225A-GFP::KanMX6::LEU2 [pRS314-MYO1-C-mCherry, CEN, TRP1] | This study |
| YO1455 | α chs2∆::His3MX6 chs2-S217D S225D-GFP::KanMX6::LEU2 [pRS314-MYO1-C-mCherry, CEN, TRP1] | This study |
| YO1528 | a chs1∆::NatMX6 chs3∆::His3MX6 [pRS314, CEN, TRP1] | This study |
| YO1535 | a chs1∆::NatMX6 chs3∆::His3MX6 [YEp-TRP1-pGAL1-HA3-CHS2, 2 μ, URA3] | This study |
| YO1536 | a chs1∆::NatMX6 chs3∆::His3MX6 [YEp-TRP1-pGAL1-HA3-CHS2-S217A, 2 μ, URA3] | This study |
| YO1537 | a chs1∆::NatMX6 chs3∆::His3MX6 [YEp-TRP1-pGAL1-HA3-CHS2-S217D, 2 μ, URA3] | This study |
| YO1543 | α chs2∆::HIS3 CDC3-mCherry::TRP1 chs2-S217A S225A-GFP::KanMX6::LEU2 [pAG426, 2 μ, URA3] | This study |
| YO1544 | α chs2∆::HIS3 CDC3-mCherry::TRP1 chs2-S217A S225A-GFP::KanMX6::LEU2 [pAG426-CYK3, 2 μ, URA3] | This study |
| YO1550 | α chs2∆::HIS3 CDC3-mCherry::TRP1 chs2-S217D S225D-GFP::KanMX6::LEU2 [pAG426, 2 μ, URA3] | This study |
| YO1551 | α chs2∆::HIS3 CDC3-mCherry::TRP1 chs2-S217D S225D-GFP::KanMX6::LEU2 [pAG426-CYK3, 2 μ, URA3] | This study |
aAll YEF and YO strains except YO1398‑1411 are derived from YEF473, YEF473A, or YEF473B.
bDerived from J230‑2D.