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. 2012 Jul 1;23(13):2605–2618. doi: 10.1091/mbc.E12-02-0090

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© 2012 Eno et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — bcl-x deficiency does not cause compensatory changes in expression of other Bcl-2 proteins. (A) Primary embryonic fibroblast cells were isolated from 10 embryos of an 11-d-pregnant bcl-x heterozygous mouse. MEFs were immortalized by expressing SV40 T antigen and cloned. Bcl-x_L expression in the established MEF cell lines was detected by Western blot analysis. (B) Changes in mRNA expression of Bcl-2 genes induced by bcl-x deficiency were determined by microarray analysis. Data are represented as fold changes in mean value of mRNA levels in two WT and two Bcl-x-KO cell lines (triplicate RNA samples for each cell line). Negative values indicate down-regulation. (C) Expression of Bcl-2 proteins in the indicated MEF cell lines was determined by Western blot analysis. KO #1, cell line 9; KO #2, cell line 4; WT #1, cell line 3; WT #2, cell line 6.