Figure 4. Phosphorylation of AQP2 at S256 in response to dDAVP, dDAVP-9r, and siRNA/dDAVP-9r polyplex treatment in IMCD cells of rat kidney.

A) IMCD suspension was treated by dDAVP (10⁻⁸ M), dDAVP-9r peptide (10⁻⁸ M), or siRNA/dDAVP-9r polyplex (1∶10) for 3 min. B) Changes of the phosphorylated AQP2 (S256) expression relative to total AQP2 were examined by semiquantitative immunoblotting (n = 4 in each group). n, number of samples which were prepared from each well.