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. 2012 Jun 28;8(6):e1002761. doi: 10.1371/journal.ppat.1002761

Figure 1. Characterization of E2 proteins expressed in HT-GPCA conditions.

Figure 1

(A) Schematic representation of the HT-GPCA method. This assay is based on the reconstitution of a luciferase activity upon co-expression of interacting partners in fusion with two inactive fragments of the Gaussia princeps luciferase (designated GL1 and GL2). The reconstituted Luciferase activity is estimated from a Normalized Luminescence Ratio (NLR) (B) 293T cells were transfected with the pTK6E2BS-Luc reporter and the GL2-E2 expressing plasmids. Fold activation is given relative to TK6E2BS-Luc in absence of E2. (C) E2-Firefly luciferase fusion proteins were expressed in 293T cells and the firefly luciferase activity was determined 24 h post-transfection. The results are expressed as a percentage of the activity obtained with the firefly luciferase only.