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. 2012 Jun 28;8(6):e1002767. doi: 10.1371/journal.ppat.1002767

Figure 8. Control of virulence factor production by LuxO inhibitors.

Figure 8

(A) Western blot analysis of TcpA (Top), HapR (middle), and LuxS (bottom, loading control) in a V. cholerae luxO D47E mutant in the presence of 0, 12.5, 25, 50, 100, and 200 µM compound 12. (B) Western blot analysis of the cytoplasmic and secreted VopD in the V. parahaemolyticus constitutively active luxO* strain (LM4476) in the presence of 0, 200, and 500 µM compound 12. An isogenic V. parahaemolyticus ΔluxO mutant (LM9968) is included as the control. (C) Cytotoxicity of V. parahaemolyticus LM4476 (luxO *) on cultured HeLa cells in the absence and presence of 500 µM compound 12. Cytotoxicity was measured by lactate dehydrogenase (LDH) release from HeLa cells. 100% cytotoxicity denotes LDH activity released upon treatment with 0.45% (v/v) Triton-X100. The V. parahaemolyticus ΔluxO mutant LM9968 and the no-bacteria control are included for comparison. Error bars represent standard errors of the mean for three independent trials.