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. 2012 Jun 28;7(6):e39586. doi: 10.1371/journal.pone.0039586

Figure 2. ER stress in neuronal cells induces the expression of pro-apoptotic proteins PUMA and BIM.

Figure 2

(A) Tunicamycin (1µM) induces a time-dependent increase in PUMA and BIM protein levels. Though AraC caused an increase in PUMA and phospho p53 protein levels, tunicamycin-induced increase in PUMA protein levels was not associated with an increase in phospho p53 levels. (B) Tunicamycin treatment also caused a robust increase in Puma mRNA levels as measured by quantitative PCR. Data points represent mean ± SEM, with n = 3.*p<0.01 by one-way ANOVA/Bonferroni post hoc test versus UT controls.