Figure 7. CHOP directly interacts with FOXO3a in response to ER stress.

(A) Nuclear and cytoplasmic fractions from untreated SH-SY5Y cells and those treated with tunicamycin (1µM) for 24h were examined for levels of total and phosphorylated FOXO3a and CHOP. GAPDH was used as a loading control for whole cell lysates or cytoplasmic fractions, while HDAC was used as a loading control for nuclear fractions (B) Cell lysates of SH-SY5Y cells treated with tunicamycin or controls were subjected to immunoprecipitation using FOXO3a polyclonal antibody or control IgG. CHOP protein was detected from immunoprecipitates by western blotting in comparison to input controls.