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. 2012 Jun 28;7(6):e39297. doi: 10.1371/journal.pone.0039297

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Wybutosine (yW) biosynthetic pathway in yeast. The yW base is located adjacent to the anticodon in tRNA^Phe. The pathway for yW biosynthesis in yeast has been described earlier [1]. (B) LC/MS analysis of nuclease P1 digested tRNA-Phe obtained from wild type (WT), the TYW2 deletion strain (ΔTYW2), and the deletion strain transformed with pYES2/hTYW2 (pYES2/hTYW2 in ΔTYW2). The panels show mass chromatograms detecting MH+ (m/z 838) of yWpA, BH2+ (m/z 377) of yW, MH+ (m/z 651) of yW-187pA, MH+ (m/z 322) of yW-187 and BH2+ (m/z 190) of yW-187, respectively.