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. 2012 Jun 28;7(6):e39950. doi: 10.1371/journal.pone.0039950

Figure 2. Effects of Nur77 overexpression and knockout on mouse AR and Kitl expression. A:

Figure 2

Nur77 +/+ mGCs were infected with Ad-LacZ or Ad-Flag-Nur77 at the indicated MOI for 48 h. AR and Kitl mRNA levels were measured by real-time PCR and shown as a ratio over control (Ad-LacZ). B: AR protein levels were measured by western blot analysis. C: Real-time PCR analysis of AR and Kitl mRNA expression in Nur77 +/+ and Nur77 −/− mGCs from 3-week-old mice. D: Western blot analysis of AR protein expression in Nur77+/+ and Nur77−/− mGCs from 3-week-old mice. The results are an average of three independent experiments performed in triplicate (*P<0.05, **P<0.01).