Figure 6. Nur77 affected the expression of the androgen signaling target gene, Kitl, by regulating AR.

KGN cells were infected with Ad-LacZ or Ad-Flag-Nur77 at 10 MOI, and Nur77 ^+/+ or Nur77 ^−/− mGCs were treated with androstenedione (A, C) or the androgen signaling antagonist, flutamide (B, D). Cellular RNA was collected, and real-time PCR analysis showed Kitl expression levels with different treatments. The results are shown as a ratio over control (n = 3). Values with different superscripts (a, b, c, d) are significantly different (p<0.05) from each other.