Fig. 3.

ERW inhibits MMP-2 and MT1-MMP gene expressions and activation of MMP-1 in HT1080 cells and H₂O₂-treated HT1080 cells. a HT1080 cells were treated with serum-free MEM containing ERW, AERW and NAC for 24 h, followed by RT-PCR analysis. b HT1080 cells were treated with H₂O₂ for 24 h and RT-PCR was done to detect MMP-2 gene expression. c HT1080 cells were treated with H₂O₂ in MEM/MQ or MEM/ERW for 24 h and RT-PCR was done to detect MMP-2 expression. d HT1080 cells were cultured in MEM supplemented with 10 % FBS overnight and then treated with MEM containing ERW for 24 h in the presence or absence of 2 μM PMS. Culture supernatants were collected and concentrated, and then zymography was performed. Densitometric analysis was performed using an ImageJ software and the relative values were shown at the upper part of each figures. The results shown are a representative of three independent experiments