Table 1.
Oligonucleotide primers for RT-PCR analysis
| Gene symbol | Gene name | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
| ACTB* | Actin, beta | ACAGCTGAGAAGGGAAATCGTG | TCCACACAGAGTACTTGCGCTC |
| HMBS* | Hydroxymethylbilane synthase | TGGAGTCTAGATGGCTCAGATAGC | CCACAAACTACTGAGGGAAAGG |
| Gck | Glucokinase | CAGAAAATGGCGGAAAATACTC | GAGATGATTCCTGCTTGAATAGTG |
| Hk1 | Hexokinase 1 | TCTAAACTCTGGGAAACAAAGG | AAGTTTGTAGAGCGTCCCATC |
| Hk2 | Hexokinase 2 | GGACTCTGTATAAGCTTCATCCTC | GTACAGGAAGTAGAGTGGGGG |
| Hk3 | Hexokinase 3 | AAGTTAAAGTATCTGGCCTTCTCC | GCAGATTTTCTTCTCCACATTC |
| Fpgt | Fucose-1-phosphate guanylyltransferase | TCAAGAGCTAGGCTTACAGTCC | GCTCAGGTTCCTCTTACTTCC |
| Fuca | Fucosidase, alpha-l- 1, tissue | AGTCTGGGAGGCAACTATCTTC | TGTCAGCTTTAGAGTCCAGGC |
| Fuk | Similar to l-fucose kinase | CTCTGGTCTTGGCACTAGC | GCTTCAGCACACTTCTCAGTC |
| Mpi | Mannose phosphate isomerase | AATTCATTGATGTGTCAACCC | AGATTGCACAATAGGGACAGG |
| GMD | Similar to GDP-mannose 4,6-dehydratase | TTTGTCATAGCTACTGGGGAAG | TAGATGCAGGGACAACACAG |
| Pmm1 | Phosphomannomutase 1 | AATGACTTTGAGATCTATGCGG | CTCCAGAGTATAAGTCCCATGC |
| Pmm2 | Phosphomannomutase 2 | GGAGTGGTAGGTGGGTCAG | CCTATGGAGAACGTGAGGC |
| Fut1 | Fucosyltransferase 1 | CCCCAGAGAAACTTCAAAGAC | TATACCTGATGTCAGCCAAATG |
| Fut8 | Fucosyltransferase 8 | TTTAGACCTGTAAGTGAGACATGC | GTGCGAGAAGCTGAAAATG |
* β-actin and hydroxymethylbilane synthase reference genes selected to calculate normalization factor with geNorm normalization (Vandesompele et al. 2002) from 10 genes