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. 2012 Jun 29;6:36. doi: 10.3389/fncir.2012.00036

Figure 2.

Figure 2

Distributions of SOM, PV, and VIP expressing cells within type I and type II nNOS immunolabeled GABAergic interneurons. (A–C) Colocalization of SOM, PV, and VIP (1) with nNOS immunolabeling (2) in GAD67:GFP knock-in mice. Arrows point to examples of type I (heavily labeled) nNOS-expressing neurons, arrowheads point to examples of type II nNOS (weakly labeled) expressing neurons. Double-labeled cells are marked by an asterisk. (D–F) Histograms reflecting the densities of SOM (green), PV (red), and VIP (blue) expressing neurons within type I (1) and type II (2) nNOS-GFP-expressing interneuron classes in cortical layers I–VI. The densities of doubled labeled cells are superimposed on the overall densities of nNOS type I (white) and nNOS type II (gray) interneurons (n = 3 mice; error bars: standard deviation; *, **, and *** indicate significant differences with p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001, respectively; roman numerals refer to neocortical layers).