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. 2012 Jun 29;2:480. doi: 10.1038/srep00480

Figure 4. Hypothermia-induced tau hyperphosphorylation is prevented by LiCl treatment in SH-SY5Y 3R-tau.

Figure 4

SH-SY5Y 3R-tau were subjected to hypothermia for 2h and treated with either LiCl or medium alone. A. Immunoblots of cell extracted proteins using several phospho-tau antibodies (AT270, CP13, AT8 Tau-1 and PHF-1). Total tau was probed using a pan tau antibody. GSK-3β inhibition was monitored by assessing both GSK-3β pS9 levels and total levels. B. Immunoblot quantifications. Ratios of phospho-epitope levels over total protein levels ±SD are represented as a percentage of hypothermic non-treated condition Ctl 30°C (Checkerboard bar). N = 3 per condition.