Fig. 2.

p47^phox−/− mice show reduced peripheral leukocyte infiltration. (a–d) Immunostaining for neutrophils in the hippocampus of p47^phox−/− and WT mice at 24 h after LPS, with 7/4 antibody. Scale bar, 100 μm. (e) Quantitation of 7/4⁺ cells showed a significant decrease of neutrophil infiltration in p47^phox−/− mice compared with WT mice. Mean ± SEM (n = 6), *p < 0.05. (f) Brain MPO protein levels were measured in supernatants from WT and p47^phox−/− mice at 24 h after LPS by ELISA. Mean ± SEM (n = 5), **p < 0.01.