Fig. 6.

Src kinase activity is required for sperm release. Stage VII–VIII rat seminiferous tubule fragments were incubated for 20 h in the presence of EtOH-vehicle (V), testosterone (T, 100 nm), flutamide alone (Flut, 1 μm), T plus either PP2 (10 nm), FSH (100 ng/ml), or PD98059 (50 μm). The number of sperm or sperm heads present in the media and after sonication of the tubule tissue was counted, and the percent sperm released into the media was determined. Values with different lowercase letters differ significantly (P < 0.05) (n = 4 to 6).