Fig. 1.

NCoRΔID mice have reduced circulating TH levels accompanied by normal serum TSH and normal TSH and TRH mRNA expression. A, Serum total T₄, free T₄, and total T₃ were measured by RIA in male adult WT and NCoRΔID mice (n = 6–10 per group). B, Serum TSH was measured in the same groups of animals by high-affinity RIA (n = 6–7 per group). C, TSH subunit mRNA levels in pituitary of male adult mice were quantified by quantitative PCR. mRNA levels are expressed relative to WT group (n = 7 animals per group). D, ISH was performed on formalin-fixed brain sections from male mice with indicated genotypes using a ³⁵S-labeled riboprobe against mouse TRH mRNA. Shown are representative images of the PVH at original magnification, ×10. The TRH mRNA expression was quantified using Image J software. Data are presented as relative pixel densities in PVH normalized to lateral hypothalamus (LH) (n = 5 animals per group). Data are presented as mean ± sem. *, P < 0.05; **, P < 0.01.