TABLE 6B.
Motor activity in the RAT (sc DMSO)
| Magnitude of Dose Tested, mg/kg-day | |||||||
|---|---|---|---|---|---|---|---|
| Author | Test | Age and route of Exposure | Age Testing | Sample Size per Dose Level (Yes/No Litter is Unit) | Measure* | 1 | 5 |
| Icenogle et al., 2004 | Figure-8 maze (1 hour with 12 5-min blocks) | GD 9–12 sc DMSO |
Wk 4–8 | 10 M +10 F; 10 litters (1 rat /sex/ reconstituted litter) Litter of origin is not unit due to randomization of pups to dams at birth. |
Habituationa (CPF × Block) | 0 | ↑ (↑ activity time block 9, 11of 12) |
| Automated | Linear trend of habituationb, | 0 | ↑ slope | ||||
| Quadratic trend of habituation∗ | 0 | 0 | |||||
| Levin et al., 2002 | Figure-8 maze (1 hour with 12 5-min blocks) Automated |
GD 1 7–20 sc DMSO |
Wk 4–6 | 10 M + 10 F; 10 litters (1 rat /sex/reconstituted litter) Litter of origin is not unit due to randomization of pups to dams at birth. |
Mean activity | 0 | 0 |
| Habituation (CPF × Block) | M0 F(Δ)d | M0 F(Δ)d | |||||
| Linear trend of habituation | M0 F↓ slope | M0 F↓ slope | |||||
| Quadratic trend of habituation* | 0 | 0 | |||||
| Levin et al., 2001 | Figure-8 maze (1 hour with 12 5-min blocks) Automated |
PND 1–4 (initial pooled analysis with PND 11–14, see below) sc DMSO |
Wk 4–6 | 10 M + 10 F; on average there was 1 rat/sex/litter Litter is not unit for initial pooled analysis combining M & F |
Mean activity | 0 | |
| Habituation (CPF × Block) | 0 | ||||||
| Levin et al., 2001 | Figure-8 maze (1 hour with 12 5-min blocks) Automated |
PND 11–14 (initial pooled analysis with PND 1–4; see above) sc DMSO |
Wk 4–6 | 10 M + 10 F; on average there was 1 rat/sex/litter Litter is not unit for initial pooled analysis combining M & F |
Mean activity | 0 | |
| Habituation (CPF × Block) | Interaction not reported | ||||||
| Linear trend of habituation | ↓ slope | ||||||
| Dam et al., 2000 | Open field (5-minute) Videotaped and blind to treatment | PND1–4 sc DMSO |
PND 21 | 5–7 pups/sex from unstated number of litters (Methods state 2 pups/sex/litter) Litter is not unit | Number of squares crossed | M↓F0 | |
| Rearing | M↓F0 | ||||||
| Self-grooming | M0 F0 | ||||||
| PND 30 | Same as for PND 21 | Number of squares crossed | M↓F0 | ||||
| Rearing | M↓F0 | ||||||
| Self-grooming | M0 F0 | ||||||
| Dam et al., 2000 | Open field (5-minute) Videotaped and Blind to treatment | PND 11–14 sc DMSO |
PND 21 | 5–7 pups/sex from unstated number of litters (Methods state 2 pups/sex/litter) Litter is not unit |
Number of squares crossed | M0 F0 | |
| Rearing | M0 F0 | ||||||
| Self-grooming | M0 F0 | ||||||
| PND 30 | Same as for PND 21 | Number of squares crossed | M0 F0 | ||||
| Rearing Self-grooming | M↑ F0 M0 F0 | ||||||
a
Habituation results reported for specific dose level if authors indicated statistically significant post-hoc intersession differences following significant dose × time interaction for the habituation curve. Otherwise, see footnote “d”
b
Linear trend of habituation was described by authors as “slope of decrease in activity over consecutive 5-minute block” so that ↑ slope could indicate less habituation or increased activity towards end of session.
d
Δ indicates a significant interaction between dose and 5-min intervals, but no post-hoc group differences reported.
*
If statistical analyses or measures were described in methods, then it was included and listed as “0” if not reported to be statistically significant