TABLE 8.

Spontaneous alternation in the RAT (s.c. DMSO)

						Magnitude of Dose Tested, mg/kg-day
Author	Test	Age and route of Exposure	Age Testing	Sample Size per Dose Level (Yes/No Litter is Unit)	Measure	1	5
Icenogle et al., 2004	Spontaneous  Alternation  T-maze Not blind	GD 9–12 s.c. DMSO	Wk 4–8	10 M +10 F; 10 litters	Spontaneous alternation	0	0
				Litter is not unit Litter of origin is not known due to randomization of pups to dams at birth	Latency	↓ Trial 1 of 5 (3 sec)	↓ Trial 1 of 5 (5 sec)
Levin et al., 2002	Spontaneous  Alternation  T-maze Not blind	GD 17–20 s.c. DMSO	Wk 4–6	10 M + 10 F; 10 litters	Spontaneous alternation	0	0
				Litter is not unit Litter of origin is not known due to randomization of pups to dams at birth	Latency	↓ Trial 1 of 5	↓ Trial 1 and 2 of 5
Levin et al., 2001	Spontaneous  Alternation  T-maze Not blind	PND1–4and PND 11–14 s.c. DMSO	Wk 8–13	10 M + 10 F; “on average there was 1 rat/sex/litter”	Spontaneous alternation	0 (PND 1–4)	0 (PND 11–14)
		analysis for both periods pooled together because no dose × period interaction		Litter is not unit for initial pooled analysis combining M & F	Latency Data pooled together in different ways	1 and 5 mg/kg-day ↓ 3 sec / trial (average) both sexes ↓ latency during 3rd session in males (1 mg/kg-day PND 1–4 data pooled together with 5 mg/kg-day PND 11–14, males and females pooled together and also analyzed for each sex separately