Figure 1.
Standardized scores representing overall levels of protein expression in 12 cell lines (blue = glioma, red = adenocarcinoma). Cells were grown in a “fed” environment including 10% FBS and harvested during the exponential growth phase before 80% confluence was reached. Scores were obtained as follows: Expression levels of proteins and phosphoproteins detected by 106 antibodies and normalized for β-actin across all cell lines were sorted and “robustified”—the highest 25% of the values received a score of 1, the lowest 25% a score of −1, and others a score of 0. The score for a cell line is the sum of the scores for its component expression values, standardized by dividing by its bootstrapped standard deviation. The F at the end of the cell line signifies fed cells.
