Table 1.
Descriptions of 108 Antibodies Used in Our RPPA Studies and Their Dilutionsa
| antibody target | catalog | source | specie | first Ab dilution | second Ab dilution | batch |
|---|---|---|---|---|---|---|
| 14-3-3ε | sc-23957 | Santa Cruz | Mouse | 1:500 | 1:15000 | 1 |
| β-Actin | A5441 | Sigma | Mouse | 1:2000 | 1:15000 | 1 |
| β-Actin | A5441 | Sigma | Mouse | 1:2000 | 1:15000 | 2 |
| ADOMET | Lab | David Feith | Rabbit | 1:500 | 1:15000 | 2 |
| AIF | sc-13116 | Santa Cruz | Mouse | 1:500 | 1:15000 | 1 |
| Akt | 9272 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Akt(Thr308) | 9275 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Akt(Ser473) | 9271 | Cell Signaling | Rabbit | 1:150 | 1:15000 | 2 |
| AMPKα | 2532 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 2 |
| AMPKα (Thr172) | 2535 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 2 |
| Antizyme inhibitor | Lab | David Feith | Rabbit | 1:500 | 1:15000 | 2 |
| Bad | 9292 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 2 |
| Bad(Ser136) | 9295 | Cell Signaling | Rabbit | 1:50 | 1:15000 | 2 |
| Bad(Ser155) | 9297 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 2 |
| Bax | 2772 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 2 |
| Bcl2 | M0887 | Dako | Mouse | 1:200 | 1:15000 | 2 |
| Bcl-xL | 2762 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 2 |
| BID | 2002 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 1 |
| Bim | 1036–1 | Epitomics | Rat | 1:200 | 1:15000 | 2 |
| Cleaved Caspase-3(Asp175) | 9664 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Cleaved Caspase-7(Asp198) | 9491 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Cleaved Caspase-9(Asp315) | 9505 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Cleaved Caspase-9(Asp330) | 9501 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Caspase-3 | 9662 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 1 |
| Caspase-8 | 9746 | Cell Signaling | Mouse | 1:250 | 1:15000 | 1 |
| β-Catenin | 9562 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 2 |
| β-Catenin(Ser33/37/Thr41) | 9561 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| CD11a(Integrin αL) | 610826 | Bd Transduction Lab | Mouse | 1:500 | 1:15000 | 2 |
| CD49b(Integrin α2) | 611016 | Bd Transduction Lab | Mouse | 1:500 | 1:15000 | 2 |
| CDC2 | CC01 | Calbiochem | Mouse | 1:200 | 1:15000 | 2 |
| CDK2 | SC-6248 | Santa Cruz | Mouse | 1:200 | 1:15000 | 2 |
| CDK4 | 2906 | Cell Signaling | Mouse | 1:200 | 1:15000 | 2 |
| cIAP-1 | 07–759 | Upstate | Rabbit | 1:200 | 1:15000 | 1 |
| Cyclin B1 | SC-245 | Santa Cruz | Rabbit | 1:200 | 1:15000 | 2 |
| Cyclin D1 | sc-718 | Santa Cruz | Rabbit | 1:1000 | 1:15000 | 1 |
| Cyclin E | sc-247 | Santa Cruz | Mouse | 1:250 | 1:15000 | 1 |
| EGFR | sc-03 | Santa Cruz | Rabbit | 1:500 | 1:15000 | 1 |
| EGFR(Tyr992) | 2235 | Cell Signaling | Rabbit | 1:50 | 1:15000 | 2 |
| Elk-1(Ser383) | 9181 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 1 |
| Erg-1/2/3 | sc-353 | Santa Cruz | Rabbit | 1:500 | 1:15000 | 1 |
| Erk 2 | sc-154 | Santa Cruz | Rabbit | 1:1000 | 1:15000 | 1 |
| Erk1/2(Thr202/Tyr204) | 9101 | Cell Signaling | Rabbit | 1:1000 | 1:15000 | 1 |
| FoxO3a | 9467 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 1 |
| FoxO3a(Ser318/321) | 9465 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 1 |
| Gab2 | 3239 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| Gab2(Tyr452) | 3882 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 2 |
| GSK-3 | 9278 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 1 |
| GSK-3α/β(Ser21/9) | 9331 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 1 |
| HDAC 3 | 2632 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 2 |
| HER2/erbB2 | 2242 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| HER2/erbB2(Tyr1248) | 06–229 | Upstate | Rabbit | 1:1000 | 1:15000 | 2 |
| HER3/erbB3 | 05–390 | Upstate | Mouse | 1:500 | 1:15000 | 2 |
| HOXA10 | AVARP13071 | Aviva systems | Rabbit | 1:500 | 1:15000 | 2 |
| HSP90 | 4875 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| JAB1 | sc-13157 | Santa Cruz | Mouse | 1:500 | 1:15000 | 2 |
| JNK1/3 | sc-474 | Santa Cruz | Rabbit | 1:200 | 1:15000 | 1 |
| JunB | 3755 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| LKB1 | 3050 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| MDM2 | sc813 | Santa Cruz | Rabbit | 1:1000 | 1:15000 | 2 |
| MEK1/2 | 9122 | Cell Signaling | Rabbit | 1:2000 | 1:20000 | 1 |
| MEK1/2(Ser217/221) | 9121 | Cell Signaling | Rabbit | 1:2000 | 1:20000 | 1 |
| c-Met(Tyr1230/Tyr1234/Tyr1235) | 44–888G | Biosource | Rabbit | 1:50 | 1:15000 | 1 |
| MSI2 | MAB10085 | Chemicon | Mouse | 1:500 | 1:15000 | 2 |
| mTOR(Ser2448) | 2971 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| c-Myc | 9402 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 1 |
| NF-kB(Ser276) | 3034 | Cell Signaling | Rabbit | 1:1000 | 1:15000 | 1 |
| NPM | 3542 | Cell Signaling | Rabbit | 1000 | 1:15000 | 2 |
| Nur77 | IMG-528 | Imgenex | Rabbit | 1:200 | 1:15000 | |
| ODC | Lab | Lisa Shantz | Rabbit | 1:100 | 1:15000 | 1 |
| p16 | sc468 | Santa Cruz | Rabbit | 1:2000 | 1:15000 | 2 |
| p21 | 2946 | Cell Signaling | Mouse | 1:250 | 1:15000 | 2 |
| p27 | SC-528 | Santa Cruz | Rabbit | 1:250 | !:15000 | 2 |
| p38 | 9212 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| p38 MAPK(Thr180/Tyr182) | 9211 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 1 |
| p53 | 554294 | BD Pharmingen | Mouse | 1:250 | !:15000 | 2 |
| p70 S6 Kinase(Thr389) | 9205 | Cell Signaling | Rabbit | 1:1000 | 1:15000 | 1 |
| PARP | 9542 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 1 |
| Cleaved PARP(Asp214) | 9546 | Cell Signaling | Mouse | 1:100 | 1:15000 | 1 |
| PDK1(Ser241) | 3061 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| PI-3 kinase | 1683–1 | Epitomics | Rabbit | 1:200 | 1:15000 | 2 |
| PKCα | 05–154 | Upstate | Mouse | 1:2000 | 1:15000 | 2 |
| PKCα(Ser657) | 06–822 | Upstate | Rabbit | 1:500 | 1:15000 | 2 |
| PKCβI | sc-8049 | Santa Cruz | Mouse | 1:300 | 1:15000 | 2 |
| PKCδ | sc937 | Santa Cruz | Rabbit | 1:200 | 1:15000 | 1 |
| PKCδ(Ser645) | 07–874 | Upstate | Rabbit | 1:1000 | 1:15000 | 1 |
| PPARγ | sc7273 | Santa Cruz | Mouse | 1:75 | 1:15000 | 2 |
| PTEN | 9552 | Cell Signaling | Rabbit | 1:1000 | 1:15000 | 2 |
| Rac1/2/3 | 2465 | Cell Signaling | Rabbit | 1:500 | 1:15000 | 2 |
| Raf-B | sc5284 | Santa Cruz | Mouse | 1:200 | 1:15000 | 2 |
| Rb | 554136 | BD Pharmingen | Mouse | 1:250 | 1:15000 | 1 |
| SHIP1 | sc-8425 | Santa Cruz | Mouse | 1:100 | 1:15000 | 1 |
| SHIP2 | 2730 | Cell Signaling | Rabbit | 1:1000 | 1:15000 | 2 |
| SIRT1 | ab32441 | Abcam | Rabbit | 1:1000 | 1:15000 | 2 |
| Smad1 | 1649–1 | Epitomic | Rabbit | 1:100 | 1:15000 | 2 |
| Smad4 | sc7966 | Santa Cruz | Mouse | 1:100 | 1:15000 | 2 |
| SPMS | Lab | David Feith | Rabbit | 1:500 | 1:15000 | 2 |
| c-Src | 44–656G | Biosource | Rabbit | 1:500 | 1:15000 | 1 |
| c-Src(Tyr527) | 2105 | Cell Signaling | Rabbit | 1:1000 | 1:15000 | 1 |
| SSAT | Lab | David Feith | Rabbit | 1:500 | 1:15000 | 1 |
| Stat1 | 9217 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Stat3 | 06–596 | Upstate | Rabbit | 1:1000 | 1:15000 | 1 |
| Stat3(Tyr705) | 9131 | Cell Signaling | Mouse | 1:1000 | 1:15000 | 1 |
| Stat5 | 9352 | Cell Signaling | Rabbit | 1:250 | 1:15000 | 1 |
| Survivin | 2802 | Cell Signaling | Mouse | 1:1000 | 1:20000 | 2 |
| TIF1 | NB100–2597 | Novus | Rabbit | 1:2000 | 1:15000 | 2 |
| YAP | 4912 | Cell Signaling | Rabbit | 1:100 | 1:15000 | 2 |
| YAP(Ser127) | 4911 | Cell Signaling | Rabbit | 1:200 | 1:15000 | 2 |
| ZNF342 | ab51265 | Abcam | Mouse | 1:2000 | 1:15000 | 2 |
a
Phosphorylation sites are in parentheses.