Table 2.
Starved-Fed Differences in Protein and Phosphoprotein Expression Detected by Antibodiesa
| protein | LNZ308 | SNB19 | U87 | U251 | LN229 | MDA231 | KM125c | OVCAR5 | MiaPaCa | MDA468 | SKOV3 | MCF7 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 14-3-3ε | 1 | 1 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 1 | 0 |
| ADOMET | 1 | 1 | 1 | 0 | 0 | −1 | 0 | 0 | 0 | 1 | −1 | −1 |
| AIF | 1 | 1 | 0 | 0 | −1 | 0 | 1 | 0 | 1 | 1 | 0 | −1 |
| Akt 1 | 1 | 0 | 0 | −1 | 0 | 0 | 1 | −1 | −1 | −1 | −1 | −1 |
| Akt(Ser473) | 1 | −1 | 1 | 1 | −1 | 1 | −1 | 0 | 0 | −1 | 0 | −1 |
| Akt(Thr308) | 1 | −1 | −1 | 1 | 1 | 0 | −1 | −1 | 0 | −1 | 0 | 0 |
| AMPKα | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | −1 | −1 | −1 |
| AMPKα(Thr172) | 1 | 1 | 1 | 0 | −1 | −1 | 0 | 1 | −1 | 0 | 0 | 0 |
| Antizyme inhibitor | −1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | −1 | 0 | −1 | 0 |
| Bad | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | −1 | −1 |
| Bad(Ser136) | 0 | 0 | 1 | 0 | 0 | −1 | −1 | −1 | 0 | 0 | −1 | −1 |
| Bad(Ser155) | 1 | 0 | 0 | 1 | 0 | 0 | −1 | 0 | −1 | −1 | 0 | −1 |
| Bax | 1 | 1 | 1 | −1 | 1 | 0 | 1 | −1 | 0 | 0 | 0 | 0 |
| Bcl2 | 1 | 1 | 1 | −1 | 1 | −1 | 1 | 1 | −1 | 0 | −1 | 1 |
| Bcl-xL | 1 | 1 | 0 | 1 | 0 | 1 | 0 | −1 | −1 | −1 | −1 | −1 |
| BID | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | −1 |
| Bim | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | −1 | 0 | 0 | −1 |
| Cleaved Caspase-3(Asp175) | 1 | 1 | −1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
| Cleaved Caspase-7(Asp198) | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
| Cleaved Caspase-9(Asp315) | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| Cleaved Caspase-9(Asp330) | 1 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | −1 | 0 | −1 |
| Caspase-3 | 1 | 1 | 0 | −1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | −1 |
| Caspase-8 | 1 | 1 | 0 | −1 | 0 | 1 | 0 | 0 | −1 | 0 | −1 | 0 |
| β-Catenin | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | −1 | −1 | −1 | 1 |
| β-Catenin(Ser33/37/Thr41) | 0 | 0 | −1 | 0 | −1 | −1 | 0 | 0 | −1 | −1 | −1 | −1 |
| CD11a(Integrin αL) | 1 | 0 | 1 | 0 | 0 | 0 | 1 | −1 | 0 | 0 | −1 | −1 |
| CD49b(Integrin α2) | 1 | 1 | 1 | 0 | 1 | −1 | 0 | 0 | −1 | 1 | −1 | 1 |
| CDC2 | 0 | 1 | 1 | 0 | 0 | 1 | −1 | 0 | −1 | −1 | −1 | −1 |
| CDK2 | 1 | 0 | 1 | 0 | 0 | −1 | 0 | −1 | −1 | 0 | −1 | 0 |
| CDK4 | 0 | 1 | 1 | −1 | 1 | 1 | −1 | 1 | 0 | 0 | −1 | −1 |
| cIAP-1 | 0 | 1 | 0 | 1 | −1 | 0 | 0 | −1 | 0 | −1 | −1 | −1 |
| Cyclin B1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | −1 | 0 | 0 | 0 | −1 |
| Cyclin D1 | 0 | 0 | 0 | 0 | −1 | −1 | 0 | −1 | 0 | 0 | 0 | −1 |
| Cyclin E | 1 | 1 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 |
| EGFR | 1 | 0 | 0 | 1 | −1 | 1 | 0 | 0 | 0 | −1 | −1 | −1 |
| EGFR(Tyr992) | 1 | 0 | 1 | 0 | −1 | 0 | 0 | 0 | 0 | 1 | −1 | −1 |
| Elk-1(Ser383) | 0 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | 1 | −1 | −1 | −1 |
| Erg-1/2/3 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | −1 | 0 | −1 | 0 |
| Erk 2 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | −1 | 0 |
| Erk1/2(Thr202/Tyr204) | 0 | 1 | 1 | −1 | 1 | −1 | 1 | 1 | −1 | 0 | 1 | −1 |
| FoxO3a | 1 | 0 | 1 | 0 | 0 | 1 | −1 | 0 | −1 | 0 | 0 | −1 |
| FoxO3a(Ser318/321) | 0 | 1 | 1 | 1 | 0 | 1 | −1 | 1 | 0 | 0 | 0 | 0 |
| Gab2 | 1 | −1 | −1 | 0 | 1 | 0 | −1 | 1 | 0 | −1 | −1 | −1 |
| Gab2(Tyr452) | 0 | 0 | 1 | 0 | 0 | 1 | −1 | 0 | −1 | 0 | 0 | −1 |
| GSK-3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | −1 | 0 | −1 |
| GSK-3α/β(Ser21/9) | 0 | −1 | −1 | −1 | 1 | −1 | 0 | −1 | 0 | −1 | 0 | 1 |
| HDAC 3 | 1 | 1 | 0 | 1 | 0 | −1 | −1 | 0 | −1 | 0 | −1 | −1 |
| HER2/ERBB2 | −1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | −1 | −1 |
| HER2/ERBB2(Tyr1248) | 1 | 1 | −1 | 1 | −1 | 0 | 1 | 0 | −1 | 0 | 0 | −1 |
| HER3/ERBB3 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | −1 | 0 | 0 | −1 |
| HOXA10 | 0 | 0 | 0 | 0 | −1 | 0 | 1 | −1 | −1 | −1 | −1 | 0 |
| HSP90 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | −1 | 0 | 1 |
| Jab1 | 1 | 1 | 1 | 0 | 0 | 0 | −1 | −1 | 0 | 0 | 0 | 0 |
| Jnk | 1 | 0 | 0 | 1 | 0 | 0 | −1 | 0 | 0 | 0 | 1 | 0 |
| JunB | 0 | 1 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | −1 | −1 |
| LKB1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | −1 | 1 |
| MDM2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | −1 | −1 |
| MEK1/2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | −1 | 0 | 0 | −1 | −1 |
| MEK1/2(Ser217/221) | 0 | 0 | 0 | −1 | 1 | −1 | 0 | 0 | −1 | −1 | 1 | −1 |
| c-Met(Tyr1230/Tyr1234/Tyr1235) | 1 | 0 | 0 | 0 | −1 | 0 | 0 | −1 | 0 | 0 | 1 | 0 |
| MSI2 | 1 | 1 | 1 | 1 | −1 | 0 | 0 | −1 | 0 | 0 | −1 | 0 |
| mTOR(Ser2448) | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| c-Myc | 0 | 0 | −1 | 0 | 0 | −1 | −1 | 0 | −1 | −1 | −1 | 0 |
| NF-kB p65(Ser276) | 1 | 1 | 0 | −1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | −1 |
| NPM | 0 | 0 | −1 | 1 | 0 | 1 | 1 | 0 | 1 | −1 | −1 | 0 |
| Nur77 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | −1 |
| ODC | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | −1 | 0 | −1 |
| p16 | 1 | 1 | 1 | 1 | −1 | 1 | 1 | 0 | 0 | −1 | 0 | −1 |
| p21 | 0 | 1 | 1 | −1 | 1 | 0 | 0 | 1 | −1 | 1 | 0 | 0 |
| p27 | 1 | 0 | 0 | 1 | 0 | −1 | 0 | 1 | −1 | 0 | −1 | 0 |
| p38 | 0 | 0 | 1 | 0 | 0 | −1 | 0 | 0 | 0 | 0 | −1 | −1 |
| p38 MAPK(Thr180/Tyr182) | 1 | 0 | −1 | 0 | −1 | −1 | 0 | 0 | 0 | −1 | 1 | −1 |
| p53 | −1 | −1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | −1 | 1 | 1 |
| p70 S6 Kinase(Thr389) | 0 | −1 | −1 | 0 | −1 | 0 | 0 | 0 | −1 | −1 | 1 | 1 |
| PARP | 1 | 1 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 |
| Cleaved PARP(Asp214) | 0 | 1 | −1 | 0 | 0 | 0 | 1 | −1 | 1 | 0 | 1 | −1 |
| PDK1(Ser241) | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| PI-3 kinase | 1 | 1 | 1 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | −1 | −1 |
| PKCα | 0 | 0 | 0 | −1 | 1 | 1 | 1 | 1 | −1 | −1 | 0 | 1 |
| PKCα(Ser657) | 1 | 1 | 1 | 1 | −1 | 0 | 0 | 1 | −1 | −1 | −1 | 1 |
| PKCβI | 1 | 1 | 0 | 1 | −1 | −1 | 0 | −1 | 0 | −1 | −1 | −1 |
| PKCδ | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | −1 | 1 | 0 | 0 |
| PKCδ (Ser645) | 1 | 0 | −1 | 0 | 0 | 0 | 0 | −1 | −1 | −1 | 0 | 0 |
| PPARγ | 0 | 0 | 1 | 0 | 0 | 1 | 0 | −1 | 0 | 0 | −1 | −1 |
| PTEN | 0 | 1 | 0 | −1 | 1 | −1 | 1 | 1 | 1 | 1 | 0 | 0 |
| Rac1/2/3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 |
| Raf-B | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | −1 |
| Rb | 0 | −1 | −1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | −1 | 1 |
| SHIP1 | 1 | 0 | −1 | 0 | −1 | 0 | −1 | 0 | 0 | −1 | 1 | −1 |
| SHIP2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | −1 | 0 |
| SirT 1 | 0 | 0 | 1 | 1 | 0 | 0 | −1 | 0 | 1 | 0 | −1 | 0 |
| Smad1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | −1 | 0 | −1 |
| Smad4 | 0 | −1 | −1 | 0 | 1 | −1 | 0 | 1 | 1 | 0 | −1 | −1 |
| SPMS | 1 | 1 | 0 | 1 | 0 | −1 | 0 | −1 | 0 | 0 | −1 | 0 |
| c-Src | 0 | 0 | 0 | 1 | −1 | 0 | 0 | 0 | 0 | −1 | −1 | 0 |
| c-Src(Tyr527) | 1 | 0 | 0 | 1 | −1 | 0 | 0 | 0 | 0 | −1 | 1 | −1 |
| SSAT | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | −1 |
| Stat1 | 1 | 1 | 1 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Stat3 | 1 | 1 | 1 | 0 | −1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Stat3(Tyr705) | 1 | 0 | 1 | 0 | 0 | 1 | −1 | 0 | 1 | 0 | −1 | −1 |
| Stat5 | 1 | 0 | −1 | −1 | −1 | 0 | 0 | −1 | 1 | 0 | 1 | −1 |
| Survivin | 1 | 0 | 0 | 0 | 1 | 0 | 0 | −1 | 0 | −1 | −1 | −1 |
| TIF1 | 1 | −1 | 0 | 0 | 0 | 1 | 0 | 1 | −1 | 0 | −1 | 0 |
| YAP | 0 | 0 | 1 | 1 | 0 | 0 | −1 | 0 | −1 | 0 | −1 | −1 |
| YAP(Ser127) | 0 | 0 | 0 | 1 | −1 | 0 | 0 | −1 | 0 | −1 | −1 | 0 |
| ZNF342 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | −1 | 0 | 0 | 0 | 0 |
| Sum Score | 60 | 40 | 26 | 16 | 2 | −2 | −3 | −8 | −19 | −28 | −35 | −45 |
a
Scores of −1, 0, or +1 were assigned depending on the quartile distribution of raw antibody difference values, where −1 denotes differences in the lowest quartile, +1 those in the highest quartile, and 0 all others.