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. Author manuscript; available in PMC: 2012 Jun 29.
Published in final edited form as: Epilepsia. 2010 Jan 13;51(8):1408–1417. doi: 10.1111/j.1528-1167.2009.02428.x

Figure 2.

Figure 2

CYP mRNAs are under the control of shear stress. (A) Schematic representation of the experimental design adopted. In the dynamic in vitro (DIV) system, media is pumped in the luminal side of polypropylene fibers, which determines shear forces. Control endothelial cells or EPI-EC (Table S1) were seeded either in the luminal side (exposed to shear stress) or in the abluminal space (no shear) of the DIV. (B–D) Exposure to shear stress significantly increased the transcript levels of the CYPs and drug transporters [(mRNA)shear/(mRNA)no-shear], suggesting a positive effect of shear stress on transcription. The comparison between mRNA levels in EPI-EC and HBMEC unveiled cell-specific differences to flow response (one-way ANOVA, *p < 0.05 HBMEC vs. EPI-EC). Results are expressed as mean ± SEM. See text for details. See also Fig. S1.