Figure 2.
Comparison of 2D NCACX spectra of MLF: top left, sampled uniformly (as a 1000 × 64 complex matrix, 4 transients per increment), and top right, nonuniformly in the indirect dimension (as a 1000 × 32 complex matrix, 8 transients per increment, using NUS1 sampling schedule). The RMS noise levels in the 2D NUS and US spectra are the same. Roman numerals I, II, and III correspond to 1D slices extracted along the direct dimensions for residues M, L, and F, respectively. Roman numerals IV, V, and VI correspond to 1D slices extracted along the indirect dimension for L-Cδ, L-Cβ, and F-CO, respectively. It can be seen that in each case the NUS dataset yields an increase in peak intensity when compared to the uniformly sampled slices displayed at the same noise level.