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Positioning of Compound 1 in the active site of FtuFabI. (A) The N3 atom of the benzimidazole ring system of Compound 1 is within hydrogen bonding distance from the 2’-hydroxyl group of NADH and the hydroxyl group of Tyr156. (B) The para-chloro atom is positioned 3.5 away from the carbonyl group of Pro154, and the methylene linker is directed towards the amide group of the nicotinamide ring in NADH.
