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. 2012 Jun 11;122(7):2590–2600. doi: 10.1172/JCI58978

Figure 2. Periostin-induced proliferation, differentiation, and activation of keratinocytes in the 3-dimensional organotypic coculture system.

Figure 2

(A) 3-dimensional organotypic coculture system. Keratinocytes were cocultured with Postn–/– or WT mouse–derived fibroblasts in the presence or absence of 10 ng/ml IL-13. (B) Histological staining with H&E, anti-CK10, and anti-CK14 of the keratinocytes cultured for 7 days. Scale bars: 25 μm. (C) Number of cells positive or negative for TUNEL, PCNA, and phospho-Akt in histological staining, counted in 10 ×200 views per section. (D) Amount of TSLP, TNF-α, GM-CSF, and IL-1α in the supernatant in keratinocytes from day 5 to day 7. (E) Mixed lymphoid reaction system. Allogeneic CD4+ T cells were cocultured with bone marrow–derived DCs treated with the supernatants from the culture as in A for 5 days. (F) Quantitative RT-PCR analysis for Il4, Il13, Ifng, and Il17a in mRNA samples prepared from allogeneic CD4+ T cells. Experiments were done at least 3 times. *P < 0.05, **P < 0.01, ***P < 0.001.