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. 2012 Jun 1;122(7):2454–2468. doi: 10.1172/JCI60842

Figure 5. Restricted clinical deficit and tissue damage in EAE in GfapCre:Vegfafl/fl mice.

Figure 5

GfapCre:Vegfafl/fl mice and Vegfafl/fl littermates (8-week-old males, at least 10 per group) were sensitized with encephalitogenic MOG35–55/CFA. (A and B) Disease was scored using a 5-point paradigm (35) and plotted (A) as a function of time and (B) by peak score. (C–F) EAE pathology, assessing for (C) astrogliosis, (D) BBB disruption, (E) inflammatory cell infiltration, and (F) demyelination and oligodendrocyte loss. (G–K) Respective morphometry of (G) VEGF-A and GFAP, as in C; (H and I) CLN-5, Ig, and fibrinogen, as in D; (J) CD45, as in E; and (K) Olig2 and demyelination (assessed by fluoromyelin), as in F. Scale bars: 50 μm (C–E); 150 μm (F); 40 μm (D and F, insets). *P < 0.05, **P < 0.01, ***P < 0.001, ANOVA plus Bonferroni test (A) or Student’s t test (B and G–K). Data are representative of 3 independent experiments.