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. 2012 Jun 11;122(7):2661–2671. doi: 10.1172/JCI61303

Figure 3. NETs are present in TRALI mouse lungs, and aspirin decreases NET formation.

Figure 3

(A–D) Representative images of NET formation detected by immunofluorescence in the lung microcirculation of (A) control mice (LPS plus isotype control mAb) and (B) mice with TRALI (LPS plus H2Kd mAb, 1.0 mg/kg), (C) with or without aspirin treatment at 2 hours after mAb injection. Platelet (CD41) staining was increased in TRALI mice and localized to areas of NET formation. Aspirin treatment decreased platelet staining to levels observed in control mice. (D) Mice treated with a higher dose of H2Kd mAb (4.5 mg/kg) had NET formation at 5 minutes after mAb injection. Scale bar: 20 μm. (E) NET formation was quantified (MPO-DNA ELISA) in mouse plasma and plotted as mean ± SD (n > 4). **P < 0.01, ***P < 0.001 versus group receiving no treatment; ††P < 0.01 versus TRALI plus DMSO group.