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. 2012 Jun 1;122(7):2637–2651. doi: 10.1172/JCI62058

Figure 10. Targeted reduction of FOXO1 in the H1650 cell line confers drug resistance to MK-2206 and erlotinib treatment.

Figure 10

(A) Western blotting for p-AKT, AKT, p-ERK, and ERK in H1650 cells after treatment with increasing doses of MK-2206. (B) Western blotting for FOXO1, BRCA1, and GAPDH after nuclear cytoplasmic fractionation of the H1650 cell line treated with 5 μM MK-2206. BRCA1: nuclear fraction control; GAPDH: cytoplasmic fraction control. (C) qRT-PCR and Western blotting for KLF6 mRNA and protein expression levels in H1650 cells after treatment with MK-2206. (D) Western blotting for p-AKT, AKT, p-ERK, and ERK in H1650 cells after treatment with erlotinib, MK-2206, or a combination of erlotinib and MK-2206. (E) Cell cycle analysis using flow cytometry of the sub-G1 cell cycle fraction after propidium iodide staining after treatment of H1650 cells with erlotinib, MK-2206, or a combination of erlotinib and MK-2206. (F) Western blot for cleaved PARP in shNTC-H1650 and shFOXO1-H1650 cells after MK-2206 and erlotinib treatment. *P < 0.05, ***P < 0.001.graphic file with name JCI62058.f8.jpg