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. 2012 Jun 1;122(7):2637–2651. doi: 10.1172/JCI62058

Figure 3. Activated RAS signaling does not affect KLF6 expression.

Figure 3

(A) Western blot of lung tissue lysates extracted and microdissected from transgenic KrasLA2 mice to exclude obvious tumor nodules versus lung tissue lysates of WT littermates. Tumor nodules were excluded to ensure that any changes in KLF6 were not secondary to tumor formation. Western blot shows p-ERK, ERK, and KLF6 protein expression normalized to mouse tubulin. (B) p-ERK to ERK ratios determined by quantitating protein expression from A. (C) qRT-PCR of KLF6 expression in lung tissue lysates of KrasLA2 mice versus WT littermates performed as previously described. For B and C, whiskers represent the range of expression, while the horizontal line shows the median. (D) Western blot for p-ERK, ERK, KLF6, and GAPDH in the HCC827 cell line treated with 1 μM of the MEK inhibitor AZD6244. (E) qRT-PCR for KLF6 in the HCC827 cell line treated with 1 μM AZD6244, normalized to GAPDH. ***P < 0.001.