Figure 1. Published nucleic acid substrates for the AAG, ALKBH2, and ALKBH3 enzymes.

Substrates for the AAG, ALKBH2, and ALKBH3 enzymes have been illustrated either in vitro or in vivo, including 3-methyladenine (3MeA), 7-methylguanine (7MeG), hypoxanthine (Hx), 8oxoG, 1,N⁶-ethanoadenine (EA), 1,N²-εG, 1-methylguanine (1MeG), εC, εA, 3-methylthymine (3MeT), 1-methyladenine (1MeA), and 3-methylcytosine (3MeC). The asterisk indicates that AAG binds but is unable to excise εC base lesions. Lesions listed in yellow are known substrates of bacterial AlkB; ALKBH2 or ALKBH3 have yet to be tested for the ability to excise these lesions. ds, double strand; ss, single strand.