Figure 8.

Marking-specific expression of cuticular protein genes and marking-associated cuticular nanostructure. (A) Heat map of the relative expression level of 128 cuticular protein genes. Red indicates positive values and green indicates negative values (color spectrum bar is shown to the left; N.D., not detected). Stage and marking of each sample is shown above. Cuticular protein genes were categorized by the motif indicated in the right. See also Figure 2. (B) Spatial expression patterns of cuticular protein genes' mRNA in P. xuthus larva during the fourth molt. Numbers in each panel indicate molt stage-hours after head capsule slippage. (C) Cuticular nanostructure (scanning electron microscopy image) of eyespot region. Magnified view of red square regions is shown to the right. (D) Cuticular nanostructure (scanning electron microscopy image) of V-shaped marking region. Magnified view of red square regions is shown to the right.