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| Dmax (Å) | k s (s−1) | ||
| Ca2+-free | Ca2+-bound | ||
| WT | 130±1 | 107±1 | 0.58±0.02 |
| E454K | 103±2 | 105±2 | 0.73±0.007 |
| 7A | 133±1 | 130±2 | ∼17 (ref 18) |
| R532A | 135±2 | 135±2 | 3.61±0.09 |
Ca2+ -bound and -free refers to SAXS measurements performed in the presence of 10 mM CaCl2 or EDTA, respectively. k s values are the mean ± SEM from 6 independent measurements derived from stopped-flow experiments and represent the slowest rate constant measured.
