Figure 2. The diffuse red fluorescence (NAB35-DsRed.T3) accumulated in the vacuole is delivered directly from the nucleus.

Wild type (BY4741) and atg6Δ cells expressing cyto-GFP alone (A), or co-expressing cyto-GFP and NAB35-DsRed.T3 (B), under growing (SS+D) and nitrogen starvation conditions (SD-N) were sampled after 24 hours. Staining with the vacuolar lumen staining dye, CMAC-Arg was used to confirm delivery of fluorescent reporters to the vacuole.