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. 2012 Jun 29;7(6):e40013. doi: 10.1371/journal.pone.0040013

Figure 6. The deletion of the ATG6 or ATG11 genes does not abrogate the delivery of n-Rosella to the vacuole.

Figure 6

(A) atg11Δ cells expressing n-Rosella were imaged under growing (SS+D) and nitrogen starvation (SD-N) conditions (0, 24, 48 and 72 hours after commencement of nitrogen starvation). (B) atg11Δ cells co-expressing both nuclear reporters were imaged under growing (SS+D) and nitrogen starvation (SD-N) conditions and the percentage of cells showing accumulation of fluorescence over time determined: ♦ green (Nvj1p-EYFP) only; • red (NAB35-DsRed.T3) only; ▾ green and red. (C) Wild type, atg6Δ, atg11Δ, atg8Δ and atg10Δ cells expressing n-Rosella were starved in SD(-N) medium for 0 and 24 hours, and degradation product (free GFP) levels were monitored by immunoblotting as described in Materials and Methods. Cytosolic PGK was detected as a loading control. *indicates the presumptive degradation product of n-Rosella lacking the NAB35 nuclear targeting signal.