Skip to main content
. 2012 Jun 29;7(6):e40013. doi: 10.1371/journal.pone.0040013

Figure 7. Deletion of the ATG3 gene abrogates the delivery of n-Rosella to the vacuole and influences nuclear morphology.

Figure 7

(A) atg3Δ and (B) atg3Δ + ATG3 cells expressing n-Rosella were imaged under growing (SS+D) and nitrogen starvation (SD−N) conditions (24 hours after commencement of nitrogen starvation). (C) Electron microscopy images of wild type (BY4741), atg3Δ and atg3Δ + ATG3 cells expressing n-Rosella were imaged under growing (SS+D) and nitrogen starvation (SD-N) conditions. Nitrogen-starved cells were sampled for imaging after 12 and 24 hours. Examples of contacts between the nucleus and vacuoles as well as alterations in nuclear morphology are presented. N, nucleus; V, vacuole. Bar, 2 µm. (D) Wild type, atg3Δ and atg4Δ cells expressing n-Rosella were starved in SD(−N) medium for 0 and 24 hours, and the levels of free GFP degradation product monitored by immunoblotting as described in Materials and Methods. Cytosolic PGK was detected as a loading control. *indicates the presumptive degradation product of n-Rosella lacking the NAB35 nuclear targeting signal. **indicates non-specific degradation product observed only in growing cells (0 hours).